Circular RNA expression profile of pancreatic ductal adenocarcinoma revealed by microarray

Circular RNA expression profile of pancreatic ductal adenocarcinoma revealed by microarray. manifestation with age; Fisher’s exact 6-O-2-Propyn-1-yl-D-galactose test was used to evaluate the circRHOT1 manifestation with sex, tumour stage and lymphatic metastasis; Mann\Whitney test was applied to evaluate the circRHOT1 manifestation with tumour size. * P?Rabbit polyclonal to PON2 used to detect cell invasion capacities in PANC\1 cells after transfection. G, Circulation cytometric assays were used to detect apoptosis of PANC\1 cells after transfection. H, Circulation cytometric assays were used to observe the cell cycle after transfection.* P < .05, ** P < 6-O-2-Propyn-1-yl-D-galactose .01 3.3. MiR\125a\3p has a important part in regulating the biological function in PANC\1 cells By using TargetScan, miR\125a\3p was shown to have a binding site for circRHOT1 (Number?3A). Then, the manifestation levels of miR\125a\3p in HPDE and PANC\1 cells were examined by using RT\qPCR. The results indicated the manifestation level of miR\125a\3p in PANC\1 cells was significantly decreased relative to that of HPDE cells (Number?3B). To investigate the function of miR\125a\3p in PANC\1 cells, a miR\125a\3p mimic and an inhibitor were used to regulate the manifestation of miR\125a\3p. The RT\qPCR results showed the effectiveness of the miR\125a\3p mimic and inhibitor (Number?3C). Overexpression of miR\125a\3p reduced cell proliferation (Number?3D,E), reduced the colony\forming capacity (Number?3F) and suppressed the invasive potential of PANC\1 cells relative to the control cells (Number?3G); however, the opposite was true for the miR\125a\3p inhibitor. In addition, flow cytometry shown that up\controlled miR\125a\3p advertised apoptosis (Number?3H) and reduced the number of PANC\1 cells arrested in S phase (Number?3I). In contrast to the miR\125a\3p mimic group, decreased miR\125a\3p reduced the apoptosis rate (Figure?3H) and induced S phase arrest in PANC\1.