Supplementary MaterialsS1 Fig: Phosphorylation of LIN-5 controls post-embryonic divisions in the

Supplementary MaterialsS1 Fig: Phosphorylation of LIN-5 controls post-embryonic divisions in the vulva. and two-cell embryos.(TIF) pgen.1006291.s005.tif (1.7M) GUID:?ADD6C2C3-28FD-499C-A99C-C8EB19A12872 S6 Fig: Phenotypical analysis shows developmental problems in LIN-5 phosphomutants. (A) Averages of quantification of intestinal nuclei and P-cells plus juvenile engine neurons (P2-P10 region) by propidium iodide staining in crazy type, homozygous LIN-5 phosphorylation mutants, and homozygous phosphorylation mutant and embryos. Spindle rotation was quantified by live-imaging of the marker. Wt shows quantity of embryos with crazy type rotation, defective A-P shows quantity of embryos with a failure to fully align in the anterior-posterior direction, defective L-R shows quantity of embryos with a failure to rotate in the left-right direction.(TIF) pgen.1006291.s006.tif (513K) GUID:?129AF539-0D81-422B-8F2B-C881C4961660 S7 Fig: Localization of LIN-5 and GPR-1 in phosphorylation mutants. Immunohistochemical staining of embryos expressing crazy type or phosphomutant and endogenously tagged RNAi. Immunohistochemical staining of heterozygous and homozygous embryos with anti-LIN-5 (reddish) and anti-GFP (green) antibodies, DNA stained with DAPI. Two representative embryos are demonstrated for each and every condition. All NBQX inhibition images same objective and magnification, anterior to the left, level bars 10 m.(TIF) pgen.1006291.s008.tif (6.2M) GUID:?1935CDB2-5000-4FF1-9B9E-47A27F04A66D S9 Fig: Cortical localization of mCherry::DHC-1 after nocodazole treatment. (A) Representative snapshots NBQX inhibition of live imaging of GFP::tubulin in one-cell embryos treated with or without RNAi + 1 M nocodazole, and imaged by spinning disk confocal microscopy. Level bars, 10 m, all images with same objective and magnification. (B) Representative snapshots of live imaging of mCherry::DHC-1 in one-cell embryos in prophase and metaphase treated with or without RNAi in the presence or absence of 1 M nocodazole, and imaged by spinning disk confocal microscopy. All images taken with same objective and magnification, anterior to the left, level bars 10 m.(TIF) FGF6 pgen.1006291.s009.tif (5.7M) GUID:?E5E38052-0768-4514-A224-D3A555C76066 S1 Video: Time-lapse imaging of developmental hallmarks in embryos. Video of DIC time-lapse microscopy imaging of hallmarks of the 1st 2 embryonic divisions inside a crazy type embryo as quantified in Fig 4C and S6 Fig. Time intervals between frames 5 s, framework rate video 7 fps. Anterior to the left.(MP4) pgen.1006291.s010.mp4 (8.9M) GUID:?40100D0C-6343-43C8-87D6-796999FFBA1C S2 Video: Spindle severing of control embryos. Representative video of UV laser spindle ablation experiments inside a embryo as quantified in Fig 4D. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s011.avi (913K) GUID:?B508A5B7-113A-45D1-B13F-EE44CCE5C046 S3 Video: Spindle severing of embryo as quantified in Fig 4D. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s012.avi (1.7M) GUID:?00BA6D84-4D5B-4BC1-A05A-343E8ECD257E S4 Video: Spindle severing of embryo as quantified in Fig 4D. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s013.avi (1.0M) GUID:?85631E84-4119-49F6-9250-1F5C9EB8D918 S5 Video: Spindle NBQX inhibition severing of embryo as quantified in Fig 4D. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s014.avi (1.8M) GUID:?D09547B7-688E-40A3-9C80-35DB82506CF1 S6 Video: Spindle severing of embryo as quantified in Fig 4D. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s015.avi (1.5M) GUID:?1749407E-1B13-42A3-8CFC-391FDE7E9919 S7 Video: Spindle severing of embryo as quantified in Fig 4E. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s016.avi (3.0M) GUID:?EA38BD94-34F3-4DF0-8DB0-F40E3172B243 S8 Video: Spindle severing of embryo as quantified in Fig 4E. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s017.avi (1.5M) GUID:?70F24D2B-6AA3-436B-B3CE-E4C244D67B86 S9 Video: Spindle severing of embryo as quantified in Fig 4E. Time intervals between frames 500 ms, framework rate video 5 fps. Anterior to the left.(AVI) pgen.1006291.s018.avi (1.0M) GUID:?104B596A-8D94-4476-BAB2-5A8623632B0F S10 Video: Meiotic spindle localization of tubulin in crazy type embryos. Representative video of GFP::tubulin inside a embryo as analyzed in Fig 7B. Time intervals between frames 10 s, framework rate video 4 fps. Anterior to the left.(AVI) pgen.1006291.s019.avi (246K) GUID:?088230F1-56EB-42D8-8C10-F7E79C0951C5 S11 Video: Meiotic spindle localization of dynein in wild type embryos. Representative video of mCherry::DHC-1 inside a embryo as analyzed.

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