ENCODE. analyses. elife-43738-fig1-data1.xlsx (10K) DOI:?10.7554/eLife.43738.005 Figure 2source data 1: Source data for STRA8 binding at promoters. elife-43738-fig2-data1.xlsx (9.3K) DOI:?10.7554/eLife.43738.011 Figure 3source data 1: Source data for RNA-seq and ChIP-seq analyses. elife-43738-fig3-data1.xlsx (10K) DOI:?10.7554/eLife.43738.014 Figure 4source data 1: Source data for Figure 4 panels. elife-43738-fig4-data1.xlsx (12K) DOI:?10.7554/eLife.43738.018 Determine 5source data 1: Source data for Determine?5?analyses. elife-43738-fig5-data1.xlsx (9.9K) DOI:?10.7554/eLife.43738.020 Physique 6source data 1: Source data for CNCCTCAG?motif enrichment at meiotic genes. elife-43738-fig6-data1.xlsx (11K) DOI:?10.7554/eLife.43738.024 Supplementary file 1: Relevant gene lists generated by this study. elife-43738-supp1.xlsx (474K) DOI:?10.7554/eLife.43738.027 Supplementary file 2: MGC33570 STRA8 ChIP-seq status, RNA-seq data, and CNCCTCAG motif count for all those protein-coding genes. elife-43738-supp2.xlsx (3.4M) DOI:?10.7554/eLife.43738.028 Supplementary file 3: STRA8 ChIP-seq status and RNA-seq data for all those meiotic prophase genes listed in Soh et al. (2015). elife-43738-supp3.xlsx (22K) DOI:?10.7554/eLife.43738.029 Supplementary file 4: Sequences used to generate the phylogenetic tree. elife-43738-supp4.xlsx (9.7K) DOI:?10.7554/eLife.43738.030 Supplementary file 5: ENCODE datasets used in this study. elife-43738-supp5.xlsx (12K) DOI:?10.7554/eLife.43738.031 Supplementary file 6: Primer and oligonucleotide sequences used in this research. elife-43738-supp6.xlsx (12K) DOI:?10.7554/eLife.43738.032 Transparent reporting form. elife-43738-transrepform.docx (247K) DOI:?10.7554/eLife.43738.033 Data Availability StatementThe ChIP-seq and RNA-seq data generated with this research can be found at NCBI Gene Manifestation Omnibus (accession quantity “type”:”entrez-geo”,”attrs”:”text”:”GSE115928″,”term_id”:”115928″GSE115928). Gene lists generated with this PSI-352938 scholarly research, including lists of genes indicated at meiotic initiation differentially, STRA8-destined genes, and STRA8-triggered genes can be purchased in Supplementary document 1. RNA-seq outcomes and STRA8 binding position for many protein-coding genes can be purchased in Supplementary document 2, while will be the true amounts of CNCCTCAG promoter motifs for many genes. Data to get a meiotic prophase gene list PSI-352938 referred to previously (Soh et al., 2015) can be purchased in Supplementary document 3. The ChIP-seq and RNA-seq data generated with this research can be found at NCBI Gene Manifestation Omnibus (accession quantity “type”:”entrez-geo”,”attrs”:”text”:”GSE115928″,”term_id”:”115928″GSE115928). Gene lists generated with this research, including lists of genes differentially indicated at meiotic initiation, STRA8-destined genes, and STRA8-triggered genes can be purchased in Supplementary document 1. RNA-seq outcomes and STRA8 binding position for many protein-coding genes can be purchased in Supplementary document 2, as will be the amounts of CNCCTCAG promoter motifs for many genes. Data to get a meiotic prophase gene list referred to previously (Soh et al., 2015) can be purchased in Supplementary document 3. Source documents have already been offered for Numbers 1-6. The next dataset was generated: Kojima ML, Web page DC. 2018. Characterization of molecular adjustments at meiotic initiation in mice. NCBI Gene Manifestation Omnibus. GSE115928 The next previously released datasets were utilized: Merkin JJ, Burge CB. 2012. Evolutionary dynamics of isoform and gene regulation PSI-352938 in mammalian tissues. NCBI Gene Manifestation Omnibus. GSE41637 Ren B. 2012. H3K4me1 ChIP-seq on PSI-352938 8-week mouse testis. ENCODE. ENCSR000CCV Snyder M. 2011. E2F4 ChIP-seq on mouse CH12 made by the Snyder laboratory. ENCODE. ENCSR000ERU Snyder M. 2011. E2F4 ChIP-seq on mouse MEL made by the Snyder laboratory. ENCODE. ENCSR000ETY Wold B. 2011. E2F4 ChIP-seq on mouse C2C12 differentiated for 60 hours. ENCODE. ENCSR000AII Snyder M. 2016. E2F1 ChIP-seq on human being K562. ENCODE. ENCSR563LLO Farnham P. 2011. E2F1 ChIP-seq on human being HeLa-S3. ENCODE. ENCSR000EVJ Snyder M. 2017. FOXM1 ChIP-seq on human being K562. ENCODE. ENCSR429QPP Snyder M. 2017. FOXM1 ChIP-seq on human being HEK293T. ENCODE. ENCSR831EIW Abstract The germ range provides the mobile link between decades of multicellular microorganisms, its cells getting into the meiotic cell routine only one time each generation. Nevertheless, the systems governing this initiation of meiosis remain understood poorly. Here, we analyzed cells going through meiotic initiation in mice, and we discovered that initiation requires the dramatic upregulation of the.
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